Study on protective immunity induced by recombinant sars-cov-2 s1 and s protein vaccine

To evaluate the immune protection of recombinant SARS-CoV-2 S1 and S protein vaccine. Methods;Recombinant SARS-CoV-2 S1 or S protein combined with aluminum hydroxide adjuvant was inoculated at different doses of 0.1 μg, 1 μg, 5 μg and 10 μg per mouse for 6-8 weeks. Serum IgG antibody liters were detected by enzyme linked immunosorbent assay (ELISA) after second immunization. The serum neutralizing antibody titers of the immunized mice against pseudotype SARS-CoV-2-Fluc WT, B. 1.1. 7, P. 1, B. 1.617.2, B. 1.621, 501Y. V2-1 strains were compared by pseudovirus neutralization test. The cellular immune levels of sera were detected by enzyme-linked immunospot assay (ELISpot). Results;Both SARS-CoV-2 S and S1 proteins could induce strong IgG antibody levels in mouse model. The sera of mice immunized with S1 protein showed obvious neutralization activity against SARS-CoV-2-Fluc WT, B. 1. 1.7 and P. 1. The sera of mice immunized with the recombinant S protein also showed obvious neutralization activity against SARS-CoV-2-Fluc B. 1.617.2 in addition to SARS-CoV-2-Fluc WT, B. 1. 1.7 and P. 1. The serum of mice immunized with two kinds of proteins had the strongest neutralizing effect on SARS-CoV-2-Fluc WT. Mouse spleen cells immunized with S protein could significantly induce the production of interferon-γ (IFN--γ) and interleukin-4 (IL-4). The levels of IgG antibody, neutralizing antibody and cellular immunity induced by S protein were higher than those of S1. Conclusion;Recombinant SARS-CoV-2 S protein vaccine can induce protective immune responses. © 2022, China Biotechnology. All rights reserved.

Robust Real-time Face Tracking for People Wearing Face Masks

Due to the outbreak of the novel coronavirus (or known as COVID-19), people are advised to wear masks when they stay outdoors in many countries. This could result in difficulty for some public safety surveillance systems involving face detection or tracking. Therefore, the development of face detection and tracking algorithms for people wearing face masks is particularly important. In this paper, a real-time tracking algorithm for people with or without face masks is proposed. This algorithm is trained on public face datasets with faces without masks. Although the training does not involve face images of people wearing face masks, we show that the proposed algorithm is robust as it is able to perform well in face tracking for people wearing face masks. We also discuss the possible scenarios where the algorithm could lose track of the target when experimenting in tracking masked faces. This can motivate future research in this area.

The effects of psychological intervention on anxiety symptoms of COVID19-positive patients isolated in hospital wards.

OBJECTIVE: The study aimed to explore the effects of psychological intervention on alleviating anxiety in patients in novel coronavirus (2019-nCoV) isolation wards. PATIENTS AND METHODS: Between January 24th, 2020 and March 5th, 2020, 103 patients were studied. Among these, 32 were patients in the isolation ward of the Infectious Disease Department in Baoding Second Hospital with suspected 2019-nCoV, and 71 patients diagnosed with 2019-nCoV were in the Tangshan Infectious Disease Hospital. Of the 103 patients included, 97 cases were observed in isolation. Using a self-control study design, each patient's anxiety was scored on a self-rating anxiety scale before receiving the psychological intervention (on the 7th day of isolation) and after receiving the intervention (on the 14th day of isolation). The severity of anxiety was evaluated based on the anxiety score before receiving the intervention. The anxiety scores before and after receiving the intervention were then compared using the paired t-test, and p<0.05 was considered statistically significant. RESULTS: After receiving the psychological intervention once or twice a week, the anxiety of the patients improved significantly after one week. CONCLUSIONS: The anxiety of patients with 2019-nCoV in isolation wards can be alleviated through psychological intervention. By alleviating patient anxiety, this intervention also helps patients maintain their psychological wellbeing, which promotes rehabilitation and helps with the control of 2019-nCoV.

An analysis of the differences in anxiety and educational background among patients isolating due to COVID-19

Introduction: The present study intends to explore the relationship between anxiety and the educational background of patients in two isolation wards on day 7 of isolation during the epidemic of COVID-19. Materials and methods: Between January 24 and March 5, 2020, 32 patients admitted to the isolation ward of the Infectious Diseases Department of Baoding Second Hospital with suspected COVID-19, and 71 patients in isolation in Tangshan Infectious Disease Hospital with a confirmed diagnosis of COVID-19 were enrolled as the main research subjects, but the actual number of cases analyzed in the present study was 97. Their anxiety was measured by an anxiety scale (SAS scale) on day seven of their isolation. A t-test was used to compare the anxiety score data of the two groups, combined with a scatter plot analysis. P<0.01 was considered statistically significant. Results: There was a total of 97 patients, in two isolation wards, in the study, of whom 47 were male and 50 female. Nineteen of them had a degree above college level, while seventy-eight of them only had a college degree or lower. The results showed that there existed a correlation between the anxiety of patients in isolation during the COVID-19 epidemic and educational background, and that those with a degree above college level were more likely to develop anxiety. Conclusion: During the quarantine period, COVID-19 patients with a degree above college level were more likely to develop anxiety, and the degree of anxiety was more noticeable, while those with a lower level of education were relatively less anxious.

Traditional Chinese Medicine guidelines for coronavirus disease 2019

OBJECTIVE: To summarize the evidence from Traditional Chinese Medicine (TCM) practice in the treatment of coronavirus disease 2019 (COVID-19) and provide timely clinical practice guidance. METHODS: The guidelines were developed in accordance with the World Health Organization rapid guideline process. The evidence on TCM for COVID-19 from published guidelines, direct and indirect published clinical evidence, first hand clinical data, and expert experience and consensus were collected. The grading of recommendations assessment, development and evaluation (GRADE) method was used to grade the evidence and make the recommendations. RESULTS: Based on the available evidence, the guidelines recommended 17 Chinese medicines for COVID-19: 2 Chinese herbal granules, 7 Chinese patent medicines, and 8 Chinese herbal injections. CONCLUSION: As the literature search was conducted on March, any subsequent versions of these guidelines require an up-to-date literature review. We hope that the evidence summary in these guidelines will be helpful in global efforts to address COVID-19. (C) 2020 JTCM. All rights reserved.